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This book has been presented for the students who are offering paramedical courses, such as BSc Nursing, PBsc and Msc Nursing. However, other students who are interested in studying the course optometry, physiotherapy and homeopathy can refer this text book to produce better results. This text book is intended to publish only for the purpose of producing centum results during their exams. Content is separated in to section A and section B according to syllabus framed by INC syllabus. Section A includes 5 Units whereas section B comprises of IL units and totally with 94 chapters. Section A comprises of historical perspectives, bacterial morphology, different culture methods, and immunology. Furthermore, all the remaining units listed with detailed account on Clinical microbiology, parasitology, mycology and virology respectively. Section B is detailed with adequate information of patient safety, sterilization, hand hygiene, BMW Management role of health care workers in prevention of disease, antibiotic stewardship Moreover, sincere effort has been taken to prepare the manuscript simply as far as this text book is concerned. For better understanding of chapters, more mnemonic forms (short forms) have been created and given in this text. To make this hook readable, the references have been taken from various microbiology test hooks, You Tube classes and some online notes. We strongly believe that this book will surely help the students to have been enlightened in their career by providing enriched knowledge.