This book enters the 37th year of its life this year after five successful editions and several more reprints. Continued popularity of the book is the reason for bringing out the present edition which features thoroughly updated text and figures. Some new material and illustrations have been added while revising and updating this edition. All the competencies listed under the CBME Guidelines | Competency Based Undergraduate Curriculum for the Indian Medical Graduate prescribed by the National Medical Commission (erstwhile Medical Council of India) have been covered. The book continues to retain its essence of giving a complete coverage of practical biochemistry syllabus for 1st MBBS in India, attempting to omit nothing essential and to include nothing superfluous. Each chapter begins with a brief theoretical background pertinent to the experiments that follow. The experiments are described in terms of purpose, reagents, procedure, principle, and practical or clinical applications. Interpretation of the findings has been discussed where relevant.